Walker Bay Nature Reserve is a nature reserve made up of five areas located on the coast between Hermanus and Cape Agulhas, in the Overberg region in the Western Cape province of South Africa. It is administered by CapeNature. The largest area, known as Walker Bay, stretches from the Klein River estuary to De Kelders at Gansbaai. Access to the reserve is via Mierkom or Walker Bay Fishing trail (through Stanford); De Kelders (Die Plaat) or Uilkraalsmond.