The Jardin Botanique de l'Université de Strasbourg (3.5 hectares), also known as the Jardin botanique de Strasbourg and the Jardin botanique de l'Université Louis Pasteur, is a botanical garden and arboretum located at 28 rue Goethe, Strasbourg, Bas-Rhin, Alsace, France. It is open daily without charge. The garden was established in 1619 for the city's Académie (which in 1621 became the University) and is thus the second oldest botanical garden in France after that of Montpellier.