Cabeza Prieta National Wildlife Refuge is located in southwestern Arizona in the United States, along 56 miles of the Mexico–United States border. It is bordered to the north and to the west by the Barry M. Goldwater Air Force Range, to the south by Mexico's El Pinacate y Gran Desierto de Altar Biosphere Reserve, to the northeast by the town of Ajo, and to the southeast by Organ Pipe Cactus National Monument.