Pembina is a city in Pembina County, North Dakota, United States. The population was 512 at the 2020 census. Pembina is located 2 miles south of the Canada–US border. Interstate 29 passes on the western side of Pembina, leading north to the Canada–US border at Emerson, Manitoba and south to the cities of Grand Forks and Fargo. The Pembina–Emerson Border Crossing is the busiest between Surrey–Blaine, and Windsor–Detroit, and the fifth busiest along the Canada-United States border.