Tolhuin is a town in the province of Tierra del Fuego, Argentina. It has 9,879 inhabitants as per the 2022 census. It is located on the eastern shore of Lake Fagnano, in the southern part of the Isla Grande de Tierra del Fuego. It is the third largest settlement on the Argentine side of Tierra del Fuego after Ushuaia and Río Grande. Tolhuin was founded on October 9, 1972. The word means "like a heart" in Selk'nam language.