Clarkefield is a town in Victoria, Australia, 41 kilometres north-west of Melbourne's Central Business District, located within the City of Hume and the Shire of Macedon Ranges local government areas. Clarkefield recorded a population of 303 at the 2021 census. Clarkefield was occupied as pastoral run, and it was named after the pastoralist, Sir William Clarke. The Clarkefield hotel and stables were established in 1857 and are now listed as heritage properties.