Chambers County is a county located in the east central portion of the U.S. state of Alabama. As of the 2020 census the population was 34,772. Its county seat is LaFayette. Its largest city is Valley. Its name is in honor of Henry H. Chambers, who served as a United States Senator from Alabama. Chambers County is included in the LaGrange, GA-AL Micropolitan Statistical Area and the Atlanta–Athens-Clarke–Sandy Springs Combined Statistical Area.