La Crosse is a city in the U.S. state of Wisconsin and the county seat of La Crosse County. Positioned alongside the Mississippi River, La Crosse is the largest city on Wisconsin's western border. La Crosse's population as of the 2020 census was 52,680. The city forms the core of and is the principal city in the La Crosse–Onalaska Metropolitan Area, which includes all of La Crosse County and Houston County, Minnesota, with a population of 139,627.