Tuolumne County, officially the County of Tuolumne, is a county located in the U.S. state of California. As of the 2020 census, the population was 55,620. The county seat and only incorporated city is Sonora. Tuolumne County comprises the Sonora, CA Micropolitan Statistical Area. The county is in the Sierra Nevada region. The northern half of Yosemite National Park is located in the eastern part of the county.