Ste. Anne, or Sainte-Anne-des-Chênes, is a town in Manitoba, Canada, located about 42 km southeast of Winnipeg. The population was 2,114 in 2016, 1,524 in 2011, and 1,513 in 2011. It is known for being located on the Seine River and at the heart of the Old Dawson Trail. The town is surrounded by the Rural Municipality of Ste. Anne.