Duntisbourne Abbots is a village and civil parish located in the English county of Gloucestershire. Duntisbourne Abbots forms part of the Cotswold District. The Five Mile House is a 17th-century Grade II listed public house at Old Gloucester Road. It is on the Campaign for Real Ale's National Inventory of Historic Pub Interiors. St Peter's Church in the village was built in the 12th century, on the site of an earlier, Saxon, church.