Grayling is a city in Yukon-Koyukuk Census Area, Alaska, United States. At the 2010 census the population was 194, unchanged from 2000. Since 1977, the Athabaskan village has seen a surge of interest on odd-numbered years, when it is the site of a checkpoint during the Iditarod Trail Sled Dog Race. It is situated after the checkpoint at Anvik and before Eagle Island.