Tuskegee is a city in Macon County, Alabama, United States. General Thomas Simpson Woodward, a Creek War veteran under Andrew Jackson, laid out the city and founded it in 1833. It became the county seat in the same year and it was incorporated in 1843. It is the most populous city in Macon County. At the 2020 census the population was 9,395, down from 9,865 in 2010 and 11,846 in 2000.