Historic Jamestowne is the cultural heritage site that was the location of the 1607 James Fort and the later 17th century city of Jamestown. It is located on Jamestown Island, on the James River at Jamestown, Virginia and operated as a partnership between Preservation Virginia and the U.S. National Park Service. The site was designated Jamestown National Historic Site on December 18, 1940, and listed on the National Register of Historic Places on October 15, 1966.