Live Oak is a city and the county seat of Suwannee County, Florida, United States. The city is midway between Tallahassee and Jacksonville. As of 2020, the population recorded by the U.S. Census Bureau was 6,735. U.S. Highway 90, U.S. Highway 129 and Interstate 10 are major highways running through Live Oak. Freight service is provided by the Florida Gulf& Atlantic Railroad, which acquired most of the former CSX main line from Pensacola to Jacksonville on June 1, 2019.