Estero is an incorporated village in Lee County, Florida, United States, located directly beside the first aquatic nature preserve established in Florida: The Estero Bay Aquatic Preserve, otherwise referred to as Estero Bay Preserve State Park which is within Estero Bay, Florida. At the time of the 2010 census, Estero was an unincorporated community and census-designated place, but incorporated as a village on the last calendar date of 2014.