Adlestrop is a village and civil parish in the Cotswolds, 3 miles east of Stow-on-the-Wold, Gloucestershire, England, on the county boundary with Oxfordshire. The River Evenlode forms the southwest boundary of the parish. The village is on a stream that flows southwest to join the river. The A436 road, which links the A44 road in Oxfordshire with Stow-on-the-Wold, passes through the parish just south of the village.