Mount Olive, also spelled Mt. Olive is an unincorporated community and a census-designated place in Jefferson County, Alabama. Its location is in the suburbs of Birmingham. As of the 2010 census, the population of the community is 4,079. Mount Olive is bordered on the south and the east by the town of Gardendale, and in fact, some areas that had previously been in Mount Olive have been annexed into Gardendale over the years. Running along part of the border between Mt. Olive and Gardendale is the major expressway, Interstate 65.