Upper Slaughter is a village in the Cotswold district of Gloucestershire, England, 4 miles south west of Stow-on-the-Wold. The village lies off the A429, which is known as the Fosse Way, and is located one mile away from its twin village Lower Slaughter, as well as being near the villages Bourton-on-the-Water, Daylesford, Upper Swell and Lower Swell. As of 2021, the village had a population of 181 inhabitants, an increase of 4 from 2011. The village is built on both banks of the River Eye.