Gretna is a city in Gadsden County, Florida, United States. It is part of the Tallahassee, Florida Metropolitan Statistical Area. The majority of the population is African American, and as of the 2020 census, had 1,357 residents, down from 1,460 at the 2010 census. The city is on U.S. 90, approximately 8 miles south of the Florida-Georgia border.