Barberino di Mugello is a comune in the Metropolitan City of Florence in the Italian region Tuscany, located about 25 kilometres north of Florence. Barberino di Mugello borders the following municipalities: Calenzano, Cantagallo, Castiglione dei Pepoli, Firenzuola, San Piero a Sieve, Scarperia, Vaiano, Vernio. Sights include the Villa Medici of Cafaggiolo.