Acquanegra sul Chiese is a comune in the Province of Mantua in the Italian region Lombardy, located about 100 kilometres southeast of Milan and about 30 kilometres west of Mantua. Acquanegra sul Chiese borders the following municipalities: Asola, Bozzolo, Calvatone, Canneto sull'Oglio, Marcaria, Mariana Mantovana, Redondesco.