Gmina Zakliczyn is an urban-rural gmina in Tarnów County, Lesser Poland Voivodeship, in southern Poland. Its seat is the town of Zakliczyn, which lies approximately 25 kilometres south-west of Tarnów and 68 km east of the regional capital Kraków. The gmina covers an area of 122.55 square kilometres, and as of 2006 its total population is 12,242. The gmina contains part of the protected area called Ciężkowice-Rożnów Landscape Park.