Nicholasville is a home rule city in and the county seat of Jessamine County, Kentucky. The population was 31,490 during the 2020 U.S. census, making Nicholasville the 10th-largest settlement in the state. Since the late 20th century, Nicholasville has undergone rapid growth; the population increased 440.23% between 1970 and 2020. The city serves as both a residential area for Lexington-area commuters and as an employment and shopping center for central Kentucky.