Desoto Acres is a census-designated place in northwestern Sarasota County, Florida, United States. It is 4 miles north of downtown Sarasota and is bordered to the east by Desoto Lakes. Desoto Acres was first listed as a CDP prior to the 2020 census. The population was 720 at the 2020 census. It is part of the North Port-Bradenton-Sarasota, Florida Metropolitan Statistical Area.