Lanesboro is a city in Fillmore County, Minnesota, United States. The population was 754 at the 2010 census. It was named after F. A. Lane, an early landowner. The Root River flows through it, with a waterfall on the edge of town. The Root River State Trail and other bike paths also serve the town. An Amish community is in the area and the town is home to many unique shops and restaurants, and a new theater.