Town Line is a hamlet and census-designated place in Erie County, New York, United States. The population was 2,367 at the 2010 census. It is part of the Buffalo–Niagara Falls Metropolitan Statistical Area. The community is located on the boundary between the towns of Lancaster and Alden. Town Line is notable for having held a nonbinding vote to secede from the United States in 1861, and for having held a ceremonial vote to rejoin the United States in 1946.