Tildenville is a census-designated place and an unincorporated area in Orange County, Florida, United States. The population was 511 at the 2010 census, most of them African American. It is part of the Orlando–Kissimmee Metropolitan Statistical Area. Generally speaking, Tildenville is a narrow area alongside Orange County Road 545, south of Florida State Road 50 and north of the Stoneybrook West planned unit development. Residents utilize Winter Garden, Florida for mail delivery.